Hot Pressurized Liquid Extraction of Polyphenols from the Skin and Seeds of Vitis vinifera L. cv. Negra Criolla Pomace a Peruvian Native Pisco Industry Waste

Abstract

The pisco industry in Peru generates large amounts of grape pomace, which is a naturalsource of bioactive compounds with potential nutraceutical applications. Hot pressurized liquidextraction (HPLE) with water-ethanol solvent mixtures (20–60%) at high temperatures (100–160 ?C)was applied to recover polyphenols from the skin and seeds of a Peruvian pisco-industry grapepomace waste. At the same HPLE conditions (60% ethanol, 160 ?C), the seed fraction extractscontained ~6 times more total polyphenol and presented ~5 times more antioxidant activity than theextract from the skin fraction. The lowest ethanol concentration (20%) and the highest temperature(160 ?C) achieved the highest recovery of flavanols with 163.61 µg/g dw from seeds and 10.37 µg/gdw from skins. The recovery of phenolic acids was maximized at the highest ethanol concentrationand temperature with 45.34 µg/g dw from seeds and 6.93 µg/g dw from skins. Flavonols were onlyrecovered from the skin, maximized (17.53 µg/g dw) at 20% of ethanol and the highest temperature.The recovery of specific polyphenols is maximized at specific extraction conditions. These conditionsare the same for seed and skin extractions. This alternative method can be used in other agroindustrialwastes in order to recover bioactive compounds with potential applications in the pharmaceuticaland food industry.Keywords: polyphenols; hot pressurized liquid extraction; skin; seeds; grape pomace; pisco industry